Species:

Universal

Alternative Name:

4-HNE, HNE, 4-hydroxy-2-nonenal, 4-Hydroxynonenal

Assay Type:

Competitive

unit:

ng/mL

Standard:

40 ng/mL

Sensitivity:

0.38 ng/mL

Detection Range:

0.63-40 ng/mL

Assay Time:

1.5H

Sample Type:

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids

Research:

Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;

Test Principle:

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 4-HNE. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to 4-HNE. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.